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Bioer Technology Co Ltd lifepro thermal cycler
Lifepro Thermal Cycler, supplied by Bioer Technology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lifepro+thermal+cycler/cycler/pm30525501__es8b04389_si_001-62-6-9
Average 86 stars, based on 1 article reviews
lifepro thermal cycler - by Bioz Stars, 2026-09
86/100 stars

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Reverse Transcription:

Article Title: The Antioxidant and Chemopreventive Activity of a Nutraceutical Derived from Brassicaceae Seed Extracts for Colorectal Cancer
Article Snippet: After homogenizing the colon aliquots, total RNA was extracted using 1 mL of Tri-Reagent (Sigma-Aldrich), solubilized in RNase-free water, and treated with DNase (Applied Biosystems, Waltham, MA, USA) to eliminate any DNA. .. Reverse transcription of RNA (100–250 ng) was then performed in a Lifepro thermal cycler (Bioer Serves Life, Hangzhou, China) according to standard protocols. .. Quantitative RT-PCR was performed with the Quantum Studio 12 K Flex Real-Time PCR System using primers (Applied Biosystems) for genes involved in oxidative metabolism, detoxification pathways, inflammatory processes, or in tumor development ( ).

Article Title: Predicting sepsis using a combination of clinical information and molecular immune markers sampled in the ambulance.
Article Snippet: .. The A260/A280 ratios were above 1.7 and the RNA integrity number (RIN) values were above 7. cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA) in a LifePro Thermal Cycler (Bioer, Hangzhou, P.R. ..

Article Title: Altered insulin sensitivity and immune function in patients with colorectal cancer.
Article Snippet: Guided by blood glucose concentrations analysed every 10 min (glucose oxidase method [21], Yellow Springs Instruments Co., Ohio, USA/Biostator), the subsequent decline in blood glucose level was counteracted by a manually adjusted variable infusion both patients and controls. .. Total RNA from the whole blood cells was extracted using PAXgene system, according to manufacturer's instructions. cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) in a LifePro Thermal Cycler (Bioer, Hangzhou, P.R. ..

Article Title: A Novel Plant-Based Nutraceutical Combined with Exercise Can Revert Oxidative Status in Plasma and Liver in a Diet-Induced-Obesity Animal Model
Article Snippet: RNA was solubilized in RNase-free water and treated with DNase (Applied Biosystems, Waltham, MA, USA) to remove any DNA present in the sample. .. A total of 100–250 ng RNA was reverse transcribed according to standard protocols using a Lifepro Thermal Cycler (Bioer Serves Life, Hangzhou, China). .. Quantitative RT-PCR was performed with a Quantum Studio 12 K Flex Real-Time PCR System (Applied Biosystems) using primers for genes involved in oxidative metabolism such as sod1 (Mm01344233_g1), sod2 (Mm01313000), cat (Mm00437992), and gpx (Mm01286848).

Article Title: Argan pulp as a novel functional ingredient with beneficial effects on multiple metabolism biomarkers
Article Snippet: RNA was solubilized in Rnase-free H2O and treated with DNase (Applied Biosystems) to remove any DNA present in the sample. .. A total of 100–250 ng of RNA was reverse transcribed according to standard protocols using a Lifepro Thermal Cycler (Bioer Serves Life, China). .. Quantitative RT-PCR was performed with QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems) using primer/probes for genes involved in oxidative metabolism as Nfe2l2 (Mm00477784_m1), sod1 (Mm01344233_g1), sod2 (Mm01313000), cat (Mm00437992), gpx2 (Mm01286848), genes coding for detoxification pathways as Nqo1 (Mm00500822_g1) and gsta2 (Mm03019257_g1), and markers involved in inflammatory processes as Tnf (Mm00443258_m1), IL-1b (Mm00434228_m1) and IL-6 (Mm00446190_m1) (Applied Biosystems).

Polymerase Chain Reaction:

Article Title: Can Sb(III)-oxidizing prokaryote also oxidize As(III) under aerobic and anaerobic conditions, and vice versa?
Article Snippet: • Sb(III)-oxidizing and As(III)-oxidizing prokaryotes, SOPs and AOPs, were

Article Title: Liquid Chromatography Based Multiplex Gene Expression Analysis Using Fluorous Separation Technique with Fluorescence Detection
Article Snippet: LC grade acetonitrile was purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). .. PCR reaction was performed with LifePro thermal cycler (BIOER Technology, Beijing, China). .. Go Taq Hot Start Colorless Master Mix was purchase from Promega (Madison, WI, USA).

Amplification:

Article Title: Identification of Steps in the Pathway of Arsenosugar Biosynthesis
Article Snippet: All PCR reactions in a total volume of 50 μL contained 25 μL of DreamTaq Green PCR Master Mix (2×) (Fermentas, Thermo Fisher Scientific, Waltham, Massachusetts, USA), 80 ng of genomic DNA template, and 25 pmol of each primer. .. Amplification was carried out in a LifePro Thermal Cycler (Bioer Technology Co., Ltd, Hangzhou, China) using the following parameters: denaturation at 94°C for 3 min, followed by 10 cycles of 94°C, 30 s; annealing temperature step-down every one cycle of 1°C (from 65°C to 56°C), 30 s; 72°C, 90 s. The annealing temperature for the following 30 cycles was 56°C with denaturation and extension phases as above, and a final extension time of 6 min at 72°C. .. PCR products were analyzed by agarose gel electrophoresis, purified using Universal DNA Purification Kit (Tiangen Biotech, Beijing), and cloned into pMD19T simple vector.

Synthesized:

Article Title: Predicting sepsis using a combination of clinical information and molecular immune markers sampled in the ambulance.
Article Snippet: .. The A260/A280 ratios were above 1.7 and the RNA integrity number (RIN) values were above 7. cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA) in a LifePro Thermal Cycler (Bioer, Hangzhou, P.R. ..

Article Title: Altered insulin sensitivity and immune function in patients with colorectal cancer.
Article Snippet: Guided by blood glucose concentrations analysed every 10 min (glucose oxidase method [21], Yellow Springs Instruments Co., Ohio, USA/Biostator), the subsequent decline in blood glucose level was counteracted by a manually adjusted variable infusion both patients and controls. .. Total RNA from the whole blood cells was extracted using PAXgene system, according to manufacturer's instructions. cDNA was synthesized using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) in a LifePro Thermal Cycler (Bioer, Hangzhou, P.R. ..



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